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A new path to anti-aging
by exosome

Multiple factors lead to 

weakening cellular environment

which causes aging.

Multiple factors lead to 

weakening cellular environment

which causes aging.

Exosomes are membrane-bound extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. The multi-vesicular body (MVB) is an endosome defined by intraluminal vesicles (ILVs) that bud inward into the endosomal lumen. If the MVB fuses with the cell surface (the plasma membrane), these ILVs are released as exosomes.

In multicellular organisms, exosomes and other EVs were discovered in biological fluids including blood, urine and cerebrospinal fluid. Exosomes were also identified within the tissue matrix, coined Matrix-Bound Nanovesicles (MBV). They are also released in vitro by cultured cells into their growth medium. Since the size of exosomes is limited by that of the parent MVB, exosomes are generally thought to be smaller than most other EVs, from about 30 to 150 nm in diameter: around the same size as many lipoproteins but much smaller than cells.
What is exosome？
描述
光老化影响了细胞行为（以自由基学说的耗散理论为首），其表现就是细胞的损伤。年轻与年长人的皮肤细胞表明，细胞的退变表现为1）干细胞增殖能力下降；2）细胞爬行速度减缓。
干细胞增殖能力降低，导致损伤死亡的组织细胞无法得到足够补充，从而损失生物行为能力；细胞爬行（迁移）速度减缓，导致战区（损伤位置）细胞不能及时获得新细胞补给，影响修复速度和效率。

成纤维细胞作为人体皮肤中的主功能细胞之一，其外泌体则具有促进成纤维细胞增殖、提升伪足迁移能力的作用。成纤维细胞属于稳定细胞，其分化方向较为统一及稳定，且成纤维细胞属于基质合成细胞，便于调教与实验观测，影响成纤维细胞可一步作用于皮肤表观。因此成纤维细胞外泌体作为成纤维细胞间的信号转运通路，是理想的皮肤抗衰手段之一。

干细胞归巢是指内源或外源性干细胞在多种因素的作用下，能定向趋向性迁移，越过血管内皮细胞至靶向组织并定植存活的过程。当机体缺血、缺氧、损伤时，机体内或者外源性干细胞具有向损伤部位优势分布的特质。




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Exosome by HEROSTEM LAB is able to boost fibroblast cell growth, meanwhile facilitating cell migration. The ability of migration comes form the increase in chemokines and psuedopodium of fibroblast cells.
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Cell growth under different concentration over 48H
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Exosome
Human dermal fibroblasts, the main cell population of the dermis, gradually lose their ability to produce collagen and renew intercellular matrix with aging. One clinical application for the autologous trans-dermis injection of HDFs that has been approved by the Food and Drug Administration aims to refine facial contours and slow down skin aging. 
Why fibroblast？
Small，but powerful
Both extrinsic and intrinsic aging lead to a microenvironment with enhanced oxidative stress and inflammatory levels, as well as senescent dermal fibroblasts. During the aging process, the upregulation of MMP production and the downregulation of collagen production lead to age-related skin disorders, including weakened dermal structure and poor mechanical integrity. Scientists indicate that spheroid formation can restore the function of aged HDFs. In addition, their findings indicate that 3D HDF-Exosomes may regulate dermal fibroblasts to induce efficient collagen biosynthesis and ameliorate inflam- mation in the skin caused by UVB irradiation. The results of the miRNA profiling data herein indicate that the downregulation of miR- 196a, as well as the upregulation of miR-133a and miR-223, might contribute to the process. 

Both extrinsic and intrinsic aging lead to a microenvironment with enhanced oxidative stress and inflammatory levels, as well as senescent dermal fibroblasts. During the aging process, the upregulation of MMP production and the downregulation of collagen production lead to age-related skin disorders, including weakened dermal structure and poor mechanical integrity. Scientists indicate that spheroid formation can restore the function of aged HDFs. In addition, their findings indicate that 3D HDF-Exosomes may regulate dermal fibroblasts to induce efficient collagen biosynthesis and ameliorate inflam- mation in the skin caused by UVB irradiation. The results of the miRNA profiling data herein indicate that the downregulation of miR- 196a, as well as the upregulation of miR-133a and miR-223, might contribute to the process. 

 在光老化的裸鼠模型中，研究人员用3D微球体培养的外泌体对比了其他3个处理方式：维生素A霜；2D培养的外泌体；骨髓间充质干细胞（MSCs）外泌体。该团队比较了治疗后皮肤厚度和胶原蛋白产生的改善。结果表明，经3D培养后的外泌体处理的小鼠的皮肤厚度比未经处理的小鼠高20％，比经过MSC处理的小鼠要高5％1。此外，他们发现，用3D培养后的外泌体处理过的皮肤所产生的胶原蛋白要比MSCs处理过的皮肤高出30％。

See data
One solution to solve all
Whitening? Anti-aging? Wrinkles?
描述
Whitening case
Anti-aging case
Wrinkle case
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墨芙拉俯视
Application
Caution
·Take one solution with meso-therapy every 3 months; 
·Use hydrating mask before and after.
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·Clean face before the treatment;
·Do not use twice after opening the vial；
·Do not use if solution appears unclear and non-transparent；
·Store at -20℃, defrost at 4℃;
·Avoid laser-like treatment on the same day.
科悦欣1
Specification


COMPOSITIONrH TYPE III COLLAGEN
CAT.NOGB01.01
SPECSType R, 3mL/5mL
PURITY> 95 % BY SDS-PAGE ANALYSES. > 95 % BY HPLC ANALYSES.
MOLECULE WEIGHTAPPROX. 60kD
ENDOTOXINES    < 0.01EU/MG  
APPEARENCESCOLORLESS
USAGEFOR RESEARCH OR FURTHER MANUFACTURE USE
STORAGE-20℃

                         



                    

                          







  • Specification

  • Q&A

Composition
Exosome from Fibroblast cell
Cat. No.
MD03.2/GB01-MD03.1
3mL/5mL
Specs
Source
Fibroblast Cell
Molecule Weight
Approximately 60 kDa
Storage
-20℃
Purity
> 95 % by SDS-PAGE analyses. > 95 % by HPLC analyses.
Endotoxines
< 0.01 EU/mg
Usage
This GMP product can be for research use or further manufacturing use.
Appearance
Colorless
Caution of usage?
Make sure of no protein allergy history. Stop using if the solution shows abnormal appearance, and contact your vendor immediately. Defrost at 4℃, and use the product after it turns completely into liquid.
How to store exosome?
Because of the instability of protein, the product should be stored at -20℃ over a year; Store at -80℃ for longer term. Do not store over 14 days at 2-8℃.
How to identify exosome?
SEM, western blot and NAT particle size analysis
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1. Hu S, Li Z, Cores J, Huang K, Su T, Dinh PU, Cheng K.(2019) Needle-Free Injection of Exosomes Derived from Human Dermal Fibroblast Spheroids Ameliorates Skin Photoaging. ACS Nano, 2019.
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