| Product | Catalog No. | Specification | Shelf life | Price |
|---|---|---|---|---|
BETAGENE Vitronectin | GB13.1 | 300μg | 24 months | 3000 |
| Product | CAT. No. | Volumn | Shelf life | Price |
|---|---|---|---|---|
rhVTN | GB13.1 | 300μg | 24 months | 3000 |
Vitronectin (VTN or VN) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. In humans it is encoded by the VTN gene. Vitronectin binds to integrin αVβ3 and thus promotes cell adhesion and spreading. It also inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpins (serine protease inhibitors). It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
储存分类代码 | WGK | 闪点（F） |
10 - Combustible Solids | WGK 2 | Not Applicable |
闪点（C) | 个人防护装备 | |
Not Applicable | Dust mask type N95, Eyeshields, Gloves | |
Specs
DATA
Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
Gene ID | 7448 |
AA Sequence | A DNA sequence encoding the human VTN (Met1-Leu478) was expressed with a polyhistidine tag at the C-terminus. |
| Host | HEK293 |
Molecule Weight | VTN consists of 468 amino acids and predicts a molecular weight of 53.4 kDa. As a result of glycosylation, it migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions. |
Biological Activity | Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to Vitronectin-coated plates (10 μg/mL and 100 μL/well), approximately ＞40% cells will adhere specifically after 30 minutes at 37 ℃. |
| Purity | ＞95% by SDS-PAGE or HPLC |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Host Cell DNA | < 0.02 ng/μg of protein |
| Host Cell Protein | < 0.05% when tested by ELISA |
| Mycoplasma | Negative |
Endotoxin (LAL) | < 0.01 EU/μg |
| Sterility | Negative |
Reconstitution | Before use this product, please read the direction below carefully. 1. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. 2. Reconstitute in a sterile aqueous buffer to an appropriate concentration. 3. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability&Storage | For long term storage, the product should be stored ≤-20°C. Please avoid repeated freeze-thaw cycles after reconstitution. 1. 12 months from date of receipt, -20 to -70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended in our USER MANUAL |
| Description |
Vitronectin (VTN or VN) is a glycoprotein of the hemopexin family which is abundantly found in serum, the extracellular matrix and bone. In humans it is encoded by the VTN gene. Vitronectin binds to integrin αVβ3 and thus promotes cell adhesion and spreading. It also inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpins (serine protease inhibitors). It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy. |
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